Wildlife crime defined here as the illegal taking, disturbance, possession, trade, or movement of animals and or their derivatives, is a growing international problem that threatens the survival of many species. In the investigation of such incidents the ‘crime scene’ may range from the carcass (or parts) of an animal to terrain that encompasses topography as varied as forest or desert and which may include diverse natural and man-made structures. Often, the location of the wildlife crime scene is isolated, with few facilities for proper investigation and collection of evidence. In poorer parts of the world and in countries experiencing social unrest, these features may present particular challenges. Wildlife crime scene work is such that equipment, investigative techniques, and scientific technology all need to be appropriate to, and the best available in, the circumstances.
Effective investigation under field conditions is likely to require a combination of portable and easy-to-use laboratory equipment coupled with modern methods of data collection and information transmission. An interdisciplinary approach is essential. Biologists/naturalists and those experienced in health studies, especially epidemiology, can often usefully complement the role of the police, enforcement officials and crime scene specialists.
Wildlife crime (WLC) is an important part of the law enforcement and it involves the illegal trade in animals, plant sand their derivatives and can result in the depletion of natural resources, invasion of pest species and the transmission of diseases .The measure of crime related to wildlife is still mysterious but it is on the increase and has observable effects with the dramatic decline in many species of flora and fauna. The estimated size of the trade in wild life, and the threat to species, it would be assumed that there is investment in forensic science to aid in combating these illegal activities .The types of forensic science methods pertinent to the enforcement of wildlife legislation include: veterinary pathology, where persons skilled in this discipline perform a similar role as their human counterparts and determine cause and time of death; crime scene examination, to record and collect such as latent fingerprints and DNA, both of the animal and potential human DNA from the perpetrator  morphology/microscopy, as simple comparison of hairs, furs and feather is often the first step to determining what species is present; ballistics, in the comparison of bullets recovered from carcasses to cartridge cases found at a poaching scene and a particular firearm if seized subsequently; document examination, to determine authenticity of documents relating to the trade in species; chemical profiling, to determine possible geographical origin based on isotope ratio; and DNA analysis to determine species and potentially link to a particular individual in a similar manner as their human counter part. Therefore, it is important to realize that forensic science has many techniques that can be complementary. Forensic science has a range of tools and it is essential that the appropriate tools used to address the allegation. There are two principal issues that are addressed in wildlife crime and these transmit to the phrasing of the various types of legislations. The first being the ability to identify a particular species and the second is the ability to determine whether the biological material can be assigned with confidence to a particular individual member of that species. The present article mainly focuses on the wildlife crime and detail description of forensic science approaches i.e. Various tools and techniques those may be used as safeguard for its solution.
Forensic Approaches Concern To WLC Investigation
A paradox to the limited prosecutions is the rise in interest in the forensic community in wildlife forensic science. There have been reviews of the subject a textbook on non-human DNA and on wildlife forensic science. One thing should be kept in mind by any forensic scientist during investigation of the crime concern to the wildlife that all the general techniques i.e. Fingerprint analysis, fibre analysis, footwear, and tyre marks, questioned documents and handwriting analysis, digital forensics, human DNA analysis, firearms analysis, soil, and chemical analysis etc. Those has been used to investigate other types of crime can also be used for wildlife crime
A summary of current forensic techniques those are available till now for the investigation of wildlife crime are briefly summarized here under:
Mainly deals with the study of the structure of animals and plants and is useful for identification of species.
B) Stable Isotope Analysis
Principally based on measuring natural variation in the chemical elements present in biological samples to establish the geographical origin or the age of a sample.
C) Radiocarbon Dating
Generally used for aging samples based on levels of carbon isotopes following the start of atmospheric nuclear bomb tests. The technique can be applied to discriminate specimens that were alive before and after the 1947 convention cut off.
D) Wildlife DNA Forensics
DNA is the genetic material present in all fauna and flora and can be found in all biological samples such as timber products, hair, fur, feathers, bones, blood, ivory, horn, saliva, faeces, nails, claws, teeth etc. and therefore, it can then be used in several applications, including molecular species identification, parentage testing and individual identification.
E) Toxicology analysis (including pesticides)
Wherever any wildlife poisoning is suspected, the chemical analysis of the victim and any bait material can establish for the presence or absence of pesticide, if any, has been used.
In wildlife crime scene work, as in other areas of forensic endeavour, it is vital that the investigations are meticulous, that detailed records are kept and that proper ‘chain of custody’ (the continuity of evidence) is maintained for each item of evidence. Such a chain will minimise the chance of loss or substitution of material and helps to prove the origin and veracity of specimens or exhibits. The chain should always be as short as possible and ideally it should not include anyone who is unwilling to appear in court or is unlikely to be a reliable witness. This cannot always be determined be forehand. Wildlife cases can be contentious and divisive, especially when someone’s livelihood, freedom or reputation is at stake.
In our experience (see also Lawton & Cooper 2009), the following are usually helpful in a wildlife crime scene investigation:
• Accurate information about the circumstances of the alleged offence, preferably in the form of a written record. This should include details of the relevant legislation.
• Discussion with those involved in reporting the incident, initiating the investigation or in bringing charges. This, too, should be covered in a written statement wherever possible. Standard reporting forms(Cooper & Cooper 2007) may already be in use for instance, in the UK, where the National Wildlife crime Unit (NWCU) has produced such a form to help collate information from those who may have witnessed a possible wildlife crime offence.
• Correct identification of the species of animal(s)involved.
• A site visit to the location where the offence is alleged to have occurred (the ‘crime scene’) or the premises where (for example) live animals need to be viewed or dead animals/their derivatives can be examined.
• Immediate recording of environmental parameters, including weather data (Merck 2007).
• Proper collection, holding, transportation, storage and submission of evidence and trace evidence and the relevant accompanying statements and records. A problem when visiting crime scenes is that ‘contamination’ — the introduction of extraneous, irrelevant, material can easily occur.
• Careful documentation and record keeping, including appropriate photographic records, leading to production of a report. Ultimately, if the matter proceeds, it will be necessary to prepare evidence for, and possibly appear in, a court of law. The holding of evidence can be particularly problematic in wildlife cases, especially under field conditions. The holding of live animals that are evidence or confiscated as ‘seizures can present major difficulties, as these will need to be properly housed and provided with adequate care. Often, especially in poorer countries, facilities for holding such specimens either do not exist or are inadequate.
Specific Applications For WLC Investigation
Identification of Species
The identification of the species is a key point in many wildlife crime investigations, and it involved to determine whether a crime has taken place. Basically, two methods can be employed to identify a species:
i) Physical Inspection
From a whole specimen, or even only parts of an animal or plant it may be possible for specialists in taxonomy to identify the species present from its morphological characteristics.
ii) DNA Analysis
Where the evidence cannot be confidently identified by physical inspection, DNA can be analysed to identify the species present. Specific regions of DNA that show variation among species but are generally conserved within species are targeted. These regions of DNA are sequenced from the specimen and compared to a validated reference database of known species. The level of similarity between the specimen and reference sequences enables the species of origin to be inferred.
B) Identification of Geographic Origin
This may be important for species which are protected by varying legislation across their range, for example ivory from African Elephants is listed under CITES Appendix everywhere except in Botswana, Namibia, South Africa, and Zimbabwe where it is listed under CITES Appendix II. Again, two possible methods can be employed.
i) Stable Isotope Analysis
Stable isotope analysis is based on measuring natural variation in the chemical elements present in biological samples. Many common elements, such as hydrogen, oxygen and carbon occur in different forms, known as isotopes. The presence or relative abundance of different isotopes allows isotope profiles to be generated for individual samples, which can then be compared to each other, or to reference data. Profiles from different environments will vary due to a number of physical, geological, and biological factors. These factors may correspond to different geographic localities and therefore these profiles can be used to infer the geographical origin of a sample. Stable isotope analysis has been used in the UK to examine hydrogen isotope levels in Bramblings to determine if levels are consistent with UK captive-bred individuals or individuals taken from the wild in their Scandinavian home range.
ii) DNA Analysis
If populations of a species are sufficiently distinct from one another, it may be possible to use genetic analysis assign a sample to its population of origin. In order to carry out this analysis, genetic information from all likely source populations must be available. Genetic population assignment is now being used to identify the origins of a wide range of illegally traded species, including fish and tigers.
C) Aging Samples
In some wildlife investigation it is necessary to know the age of a sample. For example, if a rhino horn was collected prior to 1947, then its pre-dates laws prohibiting trade in rhino horn. In order to determine whether the rhino horn was legally collected prior to 1947, a form of stable isotope analysis known as radiocarbon dating can be employed. During the early part of the 1950’s atmospheric nuclear weapons testing became common and had the effect of artificially increasing the amounts of different isotopes of the element carbon, particularly the normally rare carbon 14 (14C) which had doubled in abundance by 1965. As such, rhino horn that predates this period will be expected to have a lower ratio of 14C hand more modern specimens.
D) Animal Sexing
Where the open and closed hunting season vary between males and females (e.g. deer) it is often necessary to know the gender of a specimen to determine whether an animal was legally killed. If a carcass has been prepared for sale, morphological differences between males and females are often no longer present (e.g. antlers or genetalia). DNA analysis can, however, determine the gender of the specimen.
E) Parentage Analysis (Captive Breeding Verification)
The patterns of inheritance from parent to offspring allow DNA profiles to be used to verify family relationships. The genetic variants present in the DNA profile of an individual must be represented in its putative parents. If genetic variants are observed in an individual that do not match those found in the putative parents, then the possibility of the individual being their offspring can be excluded. This method of parentage analysis has been used successfully on many occasions to challenge the captive breeding claims of people illegally laundering wild taken birds of prey. Tests are available for a number of birds of prey and other species.
F) Individual Identification
Perhaps the most powerful DNA analysis we can perform is individual DNA profiling. This technique allows investigators to link trace evidence from a suspect to a specific incident. For example, if a dead hare is found following a coursing incident, there may be traces of dog saliva on its body. The DNA from this dog saliva can then be analysed and compared to DNA from dogs believed to have been involved the coursing. If the DNA Profile from the saliva on the hare matches a particular dog, this provides strong evidence that the dog was involved in the crime.
DNA profiling works by targeting genetic markers that are highly variable within species and are therefore likely to show differences among individuals. If two samples produce different DNA profiles, the possibility that they originate from the same individual can be excluded. If two samples share the same profile, it suggests that they may come from the same individual and it is then necessary to calculate the probability that two individuals have the same profile by chance. For evidence from animal DNA profiling to be used in court, a very high level of accuracy is required. In order to achieve this, there is a large amount of validation required for any species before the DNA profiling tool can be used for forensic analysis. This tool is currently available in the UK for dogs, badgers, and several bird of prey species.
G) Toxicology Analysis
A number of pesticides may be involved in the illegal poisoning of wildlife. This may be the deliberate abuse of a product or misuse of the product, through carelessness or failure to comply with a safe code of practice. The practise of placing illegal poison baits in the open to target birds of prey, foxes, corvids, and badgers continues around the UK. These methods are indiscriminate and may result in the death of other non-target species. A relatively small number of highly toxic products have been persistently used to illegally poison wildlife. These products may be decanted from original containers into unmarked containers. Pesticides in liquid form may be injected into bait by use of a syringe.
The Government Wildlife Incident Investigation Scheme (WIIS) investigates the deaths of wildlife potentially caused by pesticide poisoning, in addition to deaths of companion pets (dogs/cats) and beneficial insects (honeybees/bumblebees). Analytical chemistry is used to detect the presence of pesticide residues in a variety of animal tissues including gut contents, vomit, faeces, blood, urine, liver, kidney, and lung, as well as in poisoned bait. Pesticide residues are extracted from the tissues band analysed to determine the type of chemical, typically groups such of compounds such as organochlorines, organophosphates, carbamates, pyrethroids, anticoagulant and other rodenticides, or individual compounds such as strychnine, paraquat, cyanide and phosphine are found to be the cause. Pesticide analysis is a proven method of identifying deaths caused by poisoning or identifying pesticides held by suspects or finding traces in syringes, vehicles, or other relevant items.
H) Forensic Veterinary Pathology
This technique aims to determine whether the death or injury of an animal was caused by human activity or due to natural influences. A forensic clinical examination of a specimen is carried out by a suitably qualified and experienced forensic practitioner. A thorough examination by the practitioner will determine the condition of the specimen, any trauma or abnormal findings, life history data (age/sex etc.) and any rings/microchips/collars or tattoos.
Radiographs are a useful method to determine internal trauma such as broken bones or bullets. Samples (blood/tissue) may be sent for further analysis. The practitioner may also visit the crime scene to assist in the collection of important evidence such as feathers, faeces, pellets, eggshells etc. Forensic veterinary pathology is particularly useful in cases where illegal killings have involved shooting, snaring, trapping, starvation, poisoning, drowning, hare coursing and badger baiting.
K) Soil Analysis
Soil evidence at a crime scene can be used to link a suspect with an offence and is normally collected by the Scenes of Crime Officer. Soil is comprised of a mixture of organic, mineral and/or synthetic components and is considered as trace evidence. As the ratios of these components can vary over a very small area it is possible to profile the characteristics of the soil at the crime scene and compare this with a soil sample found on a person’s clothes, shoes, tools or vehicle. This is done by comparing the soil colour, particle size and shape, mineralogical composition, and biological components.
Reflected light microscopes can be used to compare the particle size and shape and x-ray diffraction can be used to compare mineralogical and biological composition. Soil analysis can be useful in cases where small amounts of soil have been found on a spade thought to be involved in a badger digging incident or in the tread of a shoe of a suspected egg collector where the raided nest location is known.
Taxidermy involves preparing, preserving, and mounting the skins of deceased animals to replicate their lifelike state. It may be possible to determine the likely cause of death even once the specimen has been mounted. An examination by an experienced taxidermist can reveal external traumas such as bullet wounds or feathers damaged by the passage of shot. Some of the larger bones and the skull may be left inside a prepared taxidermy specimen to help support the internal structure. Consequently, radiographs can be used in conjunction to determine internal traumas. However, as all of the tissue and organs have been removed this does limit the causes of death that taxidermy can uncover. A taxidermist should be able to estimate the age of a specimen.
Materials used in modern taxidermy such as enamel and acrylic eyes, nylon thread and foam bodies can all be used to estimate a date. An examination of the case and style of taxidermy can also provide useful information. It is important to age a specimen as some legislation is only applicable during certain time periods. Professional and experienced taxidermists can be sourced through the Guild of Taxidermists. If specimens are found in a taxidermist’s freezer and are thought to be from illegal killings, a post-mortem and or radiography can be carried out to determine the cause of death. Birds of prey with full crops may also be indicative that the bird has been poisoned.
K) Pollen Analysis
Pollen grains are abundant in almost all environments, are very durable and may persist on surfaces and in soils for many years .The pollen produced by flowering plants and conifers, along with the spores produced by ferns are microscopic and not visually obvious trace evidence at a crime scene. However, by examining the morphology of a tiny pollen grain it is possible to identify the genus and often the species of the plant. Conducting analysis on multiple pollen grains allows for the vegetation composition of an area to be determined.
The composition of pollen grains at a crime scene can then be compared with a sample taken from a suspect’s clothes, shoes, hair, or car. The time of year that the crime took place can also be derived as some pollen is only released during certain seasons. Pollen analysis works best when the crime scene is small such as the placement of an illegal snare or destruction of a bird of prey’s nest. In both these cases pollen from the surrounding soil can be compared with pollen found on the suspect.
Investigating wildlife crime is never easy and it is also exacting in nature. Many offences take place far from urban areas where enforcement agencies are usually based and where forensic science laboratories and forensic scientific expertise are most likely to be found. Poorly equipped countries such as India face particular problems and may have very limited facilities even ex situ. Therefore, a teamwork is needed to do so. Whenever possible, a planning meeting is advisable an opening to bring everyone together and to diminish the risk of insufficient statement before the investigation commences.
A methodical plan will ensure that all those involved are responsive of the steps that will be taken, that the scene of the alleged crime is fully examined, and that potential evidence is not lost or missed during the process. It is essential to prepare well for site visits and fieldwork and to ensure that those involved are familiar with the necessary rules, SOPs, and codes of practice. This is particularly important when backup or direction from the police or other experienced people is not available.
What Is Wildlife Crime?
Ans: Wildlife crime can be defined as taking, possession, trade or movement, processing, consumption of wild animals and plants or their derivatives in contravention of any international, regional, or national legislation(s).
What Does Wildlife Forensics Do?
Ans: Wildlife forensics is the application of science to legal cases involving wildlife. This field uses scientific procedures to investigate wildlife-related crimes involving the exotic pet trade, poaching, other illegal hunting activities, and even oil spills.
What Is Radiocarbon Dating?
Radiocarbon dating is a method that provides objective age estimates for carbon-based materials that originated from living organisms. An age could be estimated by measuring the amount of carbon-14 present in the sample and comparing this against an internationally used reference standard.
What Is Taxonomy?
Ans: Taxonomy is the science of naming, describing, and classifying organisms and includes all plants, animals, and microorganisms of the world.